The Gygi lab has developed and contributed to many resources for the dissemination of large scale proteomics data, such as PhosphoMouse, BioPlex Explorer, GGBase, DepMap, and many more!
...the goal is to transform data into information and information into insight.
-Carly Fiorina
BioPlex Explorer
To enable search and download of the BioPlex Interactome datasets, we developed the web application - BioPlex Explorer. With the Explorer, users can search for their favorite protein within the BioPlex interactome and investigate subnetworks, network communities, or cell-line specific interactions.
Explore!
PhosphoMouse
To better understand the role of phosphorylation in maintenance of physiological differences among tissues, we performed proteomic and phosphoproteomic characterizations of nine mouse tissues. We identified 12,039 proteins, including 6296 phosphoproteins harboring nearly 36,000 phosphorylation sites. Comparing protein abundances and phosphorylation levels revealed specialized, interconnected phosphorylation networks within each tissue while suggesting that many proteins are regulated by phosphorylation independently of their expression.
Explore!
Reactive Cysteine Profiling - SLC-ABPP
We redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based probe, sample multiplexing, reduced protein starting amounts and software to boost data acquisition in real time on the mass spectrometer. Our method, streamlined cysteine activity-based protein profiling (SLC-ABPP), achieved a 42-fold improvement in sample throughput, corresponding to profiling library members at a depth of >8,000 reactive cysteine sites at 18 min per compound. We applied it to identify proteome-wide targets of covalent inhibitors to mutant Kirsten rat sarcoma (KRAS)G12C and Bruton’s tyrosine kinase (BTK). In addition, we created a resource of cysteine reactivity to 285 electrophiles in three human cell lines, which includes >20,000 cysteines from >6,000 proteins per line.
Explore Ligandable Cysteines!
CCLE - Proteomics
We expanded the Cancer Cell Line Encyclopedia to include quantitative proteome profiling by mass spectrometry of 375 cell lines from diverse lineages. The experiment was performed in multiplex format with 9 biological samples per plex and one common sample to normalize between plexes. We quantified an average of >9,000 proteins per experiment across 42 multiplex experiments consisting of 504 analytical LC-MS/MS runs and over 1,500 hours of instrument time! These data have been fully integrated into the DepMap Portal.
Explore CCLE Data!
Explore @ the DepMap Portal!
OxiMouse
Mammalian tissues engage in specialized physiology that is regulated through reversible modification of protein cysteine residues by reactive oxygen species (ROS). ROS regulate a myriad of biological processes, but the protein targets of ROS modification that drive tissue-specific physiology in vivo are largely unknown. Here, we develop Oximouse, a comprehensive and quantitative mapping of the mouse cysteine redox proteome in vivo. We use Oximouse to establish several paradigms of physiological redox signaling. We define and validate cysteine redox networks within each tissue that are tissue selective and underlie tissue-specific biology. We describe a common mechanism for encoding cysteine redox sensitivity by electrostatic gating. Moreover, we comprehensively identify redox-modified disease networks that remodel in aged mice, establishing a systemic molecular basis for the long-standing proposed links between redox dysregulation and tissue aging. We provide the Oximouse compendium as a framework for understanding mechanisms of redox regulation in physiology and aging.
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GGBase
To globally characterize the ubiquitin-modified proteome (ubiquitinome), we utilized a monoclonal antibody that recognizes diglycine (diGly) containing isopeptides following trypsin digestion. We identify ~19,000 diGly modified lysine residues within ~ 5000 proteins. Using quantitative proteomics we monitored temporal changes in diGly site abundance in response to both proteasomal and translational inhibition indicating both a dependence of on-going translation to observe alterations in site abundance and distinct dynamics of individual modified lysines in response to proteasome inhibition.
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Short-Lived Proteins
Rapid protein degradation enables cells to quickly modulate protein abundance. Dysregulation of short-lived proteins plays essential roles in disease pathogenesis. A focused map of short-lived proteins remains understudied. Cycloheximide, a translational inhibitor, is widely used in targeted studies to measure degradation kinetics for short-lived proteins. Here, we combined cycloheximide chase assays with advanced quantitative proteomics to map short-lived proteins under translational inhibition in four human cell lines. Among 11,747 quantified proteins, we identified 1,017 short-lived proteins (half-lives ≤ 8hr). We further quantified 103 proteins with different stabilities among cell lines. This study provides a large-scale resource of human short-lived proteins under translational arrest, leading to untapped avenues of protein regulation for therapeutic interventions.
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Founder Mouse Liver Proteome
The Diversity Outbred (DO) mouse model is a powerful genetic resource to systemically evaluate how genetic variation influences protein expression and physiological phenotype. We performed a pilot study exploring the full proteome expression of the 8 founder strains (n = 8 for each founder; four males and four females) from which the DO cohort was derived. The eight founder strains are A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/H1LtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ.